Well, you need to read/know on how to use AjaxControlToolkit controls.
It's very clear that you tried to use a PasswordStrength control. When you were using it, you have not assigned controls action/trigger connected to any other control.
For example, you want that this control checks if the given password in TextBoxA is fine or not. There can be multiple textboxes on the page. Thus, In order to tell PasswordStrength control that you want to check TextBoxA value's strength, you need to connect that to it. This connection can be done using property 'TargetControlID' of the PasswordStrength control.
Just put the property TargetControlID="txtBoxPassword" and you should be done.
Look at the sample here:
PasswordStrength Demonstration[
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