Use below mentioned code snippet
SqlConnection con = new SqlConnection("Data Source=NSYS1\\SQLEXPRESS;Initial Catalog=agfl;connect timeout=30;Integrated Security=True");
string strQuery = "select * from stv where rdate="+"'"+Textbox6.Text+"'";
SqlCommand cmd = new SqlCommand(strQuery);
SqlDataAdapter sda = new SqlDataAdapter(cmd ,con);
DataSet ds = new DataSet();
ds.clear();
sda.Fill(ds);
gvuser.DataSource = ds;
gvuser.DataBind();
For your information when using data adapter no need to provide con.open or close it will manage itself.
Hope This Helps!!!