It should not conflict (assuming that the bit in brackets is intended as a comment), each label is independant.
However, I would convert the textbox1.Text value to a numeric value once before using the converted value in each line:
Dim baseCurrency As Double
If Double.TryParse(textbox1.Text, baseCurrency) Then
label1.text = (baseCurrency * 54).ToString()
...
End If
In addition, I wouldn't use "magic numbers" for the exchange rate... Rates tend to change on a minute by minute basis, so your software will be out of date within seconds of you compiling it!