In cashbook, how to get running balance from column 1 minus column 2 and put the balance in the column 3

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Gridview

I'm currently developing a Cash Book system using ASP.NET web forms in c#. I have input text boxes for the "Date", "Transactions", "IN", "OUT", "Running Balance" and a "Button" to submit. After clicking the "Button", all entries can and will be viewed in an Item-template grid view. Now the problem is this, I don't know how to get the running total.

Below is what I needed.

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What I have tried:

Hope somebody can spare me a little bit of your time.