JW player does not play recorded video

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ASP.NET

I have recorded video by camera and it saved as mp4 but when I tried to play it on the web page by jw player I got Error loading media file could not be played

please help

What I have tried:

<script type="text/javascript">

jwplayer("aa").setup({
flashplayer: "jwplayer/player.swf",
file: "~/Videos/E-learning.mp4",

width: "550",
height: "400",

primary:'html5',
poster: 'images/notAvailble.jpg',
});

</script>