How do I add items with images on data grid one by one

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VS2008

Hi Everyone, can you help me with this please see the photo below. I want to add items with images on the datagrid from a database but only when i search the item and click add at first click on add its correct. but when i search an item again and click add it will duplicate the columns.

What I have tried:

Public Sub retrieveItems()
'Retrieve Image from database
' dgvQuotation.Columns.Clear()

Dim cmd As New MySqlCommand
Dim dr As MySqlDataReader

Try
dgvQuotation.RowTemplate.Height = 100
Dim dgvImage = New DataGridViewImageColumn()
dgvImage.HeaderText = "Image"
dgvImage.ImageLayout = DataGridViewImageCellLayout.Stretch

Dim dgvCode As DataGridViewTextBoxColumn = New DataGridViewTextBoxColumn()
dgvCode.HeaderText = "Code"

Dim dgvName As DataGridViewTextBoxColumn = New DataGridViewTextBoxColumn()
dgvName.HeaderText = "Name"
Dim dgvDesc As DataGridViewTextBoxColumn = New DataGridViewTextBoxColumn()
dgvDesc.HeaderText = "Description"
Dim dgvRetail As DataGridViewTextBoxColumn = New DataGridViewTextBoxColumn()
dgvRetail.HeaderText = "Retail"
Dim dgvTrade As DataGridViewTextBoxColumn = New DataGridViewTextBoxColumn()
dgvTrade.HeaderText = "Trade"
dgvQuotation.Columns.Add(dgvCode)
dgvQuotation.Columns.Add(dgvName)
dgvQuotation.Columns.Add(dgvDesc)
dgvQuotation.Columns.Add(dgvRetail)
dgvQuotation.Columns.Add(dgvTrade)
dgvQuotation.Columns.Add(dgvImage)

dgvQuotation.AutoSizeColumnsMode = DataGridViewAutoSizeColumnsMode.Fill
dgvQuotation.RowTemplate.Height = 120
dgvQuotation.AllowUserToAddRows = False

conDB.Close()
connectDB()
cmd.Connection = conDB
cmd.CommandType = CommandType.Text
cmd.CommandText = "select * from items where item_code='" & frmManageItem.dgvitems.SelectedCells(1).Value & "'"
dr = cmd.ExecuteReader

If Not dr Is Nothing Then
dr.Read()
Dim qcode As String
Dim qname As String
Dim qdesc As String
Dim qretail As String
Dim qtrade As String
qcode = dr("item_code").ToString
qname = dr("item_name").ToString
qdesc = dr("item_description").ToString
qretail = dr("retail_price").ToString
qtrade = dr("trade_price").ToString

Dim data As Byte() = DirectCast(dr("Photo"), Byte())
Dim ms As New MemoryStream(Data)
PictureBox2.Image = Image.FromStream(ms)
'PictureBox2.Image.Save(ms, PictureBox2.Image.RawFormat)

Dim md As MemoryStream = New MemoryStream()
PictureBox2.Image.Save(md, PictureBox2.Image.RawFormat)

Dim img As Byte() = ms.ToArray()

dgvQuotation.Rows.Add(qcode, qname, qdesc, qretail, qtrade, img)
dr.Close()

For i As Integer = 0 To dgvQuotation.Columns.Count - 1
If TypeOf dgvQuotation.Columns(i) Is DataGridViewImageColumn Then
DirectCast(dgvQuotation.Columns(i), DataGridViewImageColumn).ImageLayout = DataGridViewImageCellLayout.Stretch
End If

Next
End If
Catch ex As Exception
MessageBox.Show(ex.Message)
Finally
conDB.Close()
End Try
End Sub