How do i include folder / subfolder for updates?

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updating

Site for example, I have File.abc located at ABC\DEF\File.abc
how could I include the folder ABC and subfolder DEF on the startup path of vb.net application? So when I deploy the updates it should be ABC\DEF\File.abc...complete path. Isn't it possible?

What I have tried:

I tried to put the complete path but it doesnt work...like:

1.0.0.0|Test.exe?ABC\DEF\File.abc
1.0.0.0|Test.exe?\ABC\DEF\File.abc
1.0.0.0|Test.exe?\\ABC\\DEF\\File.abc
1.0.0.0|Test.exe?/ABC/DEF/File.abc
1.0.0.0|Test.exe?//ABC/DEF//File.abc

and many more but its not working for me...