Update Panel not updating dynamically created controls on dynamically created button click event

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C#2.0

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On dynamically created control button click event working and refreshing data on 1st button click but on multiple button click event in Panel inside dynamic panel after 1st button click update panel not refreshing dynamically created panel on delete.

<asp:UpdatePanel ID="updateitem" runat="server" UpdateMode="Conditional">

Inside panel i am creating dynamic control and inside dynamic control again dynamic control.When i am trying to delete created control inside panel through below method and i want to delete each Child panel on button click.

for better understanding follow the below example:

<asp:Panel Id="Panel1" runat="Server"> this is actual panel in design page

<asp:Panel Id="PanelD1" runat="Server"> this is dynamic created panel in code behind

<asp:Panel Id="PanelD1Child1" runat="Server"> 

this is dynamic child panel of PanelD1 in code behind 

</asp:Panel>

<asp:Panel Id="PanelD1Child2" runat="Server"> 

this is dynamic child panel of PanelD1 in code behind

</asp:Panel>

</asp:Panel>

</asp:Panel>

if (DynamicPanel.Controls.Contains(CustPanel))
{
  DynamicPanel.Controls.Remove(CustPanel);
  CustPanel.Dispose();
  updateitem.Update();
}

It works for 1st button click inside dynamically created panel but when i click another button inside same panel it follow the above method and removes control but UpdatePanel is not refreshing Panel that's why page is showing the same controls after delete button click.