Add Connection string in web.config file.
<connectionStrings>
<add name="Conn" connectionString="Data Source=Server Name;Initial Catalog=DB Name;User id=userid;Password=password;Persist Security Info=true;Pooling=true" providerName="System.Data.SqlClient"/>
</connectionStrings>
Add Namespaces in C#,
using System.Data.SqlClient;
using System.Configuration;
In your C# code, Initialize connection string.
SqlConnection con = new SqlConnection();
string cnstr = ConfigurationManager.ConnectionStrings["Conn"].ConnectionString.ToString();
con.ConnectionString = cnstr;
con.Open();
con.Close();