<pre lang="text">
You mean only one check box allowed to checked if so you can do it using JQuery in such a manner
#1:- In template Column write the checkbox ID like
<asp:checkbox id="chk_"+ID onclick="SelectOne(ID);">
ID will be PK fields which you are getting from database
funtion SelectOne(Id){
//find all the checkbox into the grid unchecked
$('input[id*="chk_"]').removeAttr("checked");
// then make checked what you have clicked
$("#chk_" + Id ).attr("checked", "checked");
}
TRY THIS WILL WORK IF ANY ISSUE WRITE ME
THANKS