Try this
DateTime time1 = DateTime.ParseExact("08:30:00", "HH:mm:ss", System.Globalization.CultureInfo.InvariantCulture);
DateTime time2 = DateTime.ParseExact("17:15:00", "HH:mm:ss", System.Globalization.CultureInfo.InvariantCulture);
TimeSpan diff = time2 - time1;
OR
DateTime time1 = DateTime.Parse("08:30:00");
DateTime time2 = DateTime.Parse("17:15:00");
TimeSpan diff = time2 - time1;