The problem is your connection string & /or server configuration.
Your connection string does not include a SQL Username & Password but instead are using Integrated Security or Trusted_Connection in your string with the value set to SSPI.
The error is advising that the SQL Server is unable to authenticate the User that is making the request.
This can be caused by several reasons for example;
a) SQL is running under a local account that cannot query the domain controller to determine if the user making the request has the required permissions
b) The account that is making the request (I would suggest this is a website due to the
ScriptManager reference) does not have permission to access the DB, probably because the website is running under a local account on your web server
Review how you are connecting to SQL & create an appropriate connection string - the below links will assist.
SqlConnection.ConnectionString Property (System.Data.SqlClient)[
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ConnectionStrings.com - Forgot that connection string? Get it here![
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Kind Regards