There's no way to disable an specific row using the datagridview object.
But there was another options, like changing the format of the row you want in order to make this seems like disabled:
Private Sub ConfigDisabled(Byval RowNumber as integer)
Dim CelaActiva As New DataGridViewCellStyle
CelaActiva.BackColor = Color.White
CelaActiva.Alignment = DataGridViewContentAlignment.MiddleCenter
CelaActiva.SelectionBackColor = Color.White
CelaActiva.SelectionForeColor = Color.Gray
CelaActiva.ForeColor = Color.Gray
Datagridview1.Rows(RowNumber).DefaultCellStyle = CelaActiva
Datagridview1.Rows(RowNumber).ReadOnly = True
End Sub
Hope this helps you ;)