image control not showing image

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C#

ASP.NET

I have two image control on my page, when i take its image url from images folder then its showing image and when i take image url from other folder(Ads_Images) then it is not showing image, Don't know whats wrong with it...

<asp:Image ID="Image1" runat="server" ImageUrl="Ads_Images/7f0e7ef3-4cb8-40e0-a835-00314768a70a70-515.jpg"/>

<asp:Image ID="Image2" runat="server" ImageUrl="Images/imagenotavailable.jpg" />

where Ads_Images and Images are two folders in my project, Images is my default folder and in Ads_Images user can store images and save path to database, I am using GUID to add with my images.

protected void btnUpload_Click(object sender, EventArgs e)
    {
        string fileName = string.Empty;
        string filePath = string.Empty;
        string getPath = string.Empty;
        string pathToStore = string.Empty;
        if (FileUpload1.PostedFile != null)
        {
            fileName = FileUpload1.FileName;
            filePath = Server.MapPath("Ads_Images/" + System.Guid.NewGuid() + fileName);
            FileUpload1.SaveAs(filePath);
            SqlCommand cmd = new SqlCommand("insert into userimage (FileName, FilePath)" + " values(@FileName, @FilePath)", con);
            cmd.Parameters.AddWithValue("@FileName", fileName);
            int getPos = filePath.LastIndexOf("\\");
            int len = filePath.Length;
            getPath = filePath.Substring(getPos, len - getPos);
            pathToStore = getPath.Remove(0, 1);
            cmd.Parameters.AddWithValue("@FilePath", pathToStore);
            try
            {
                con.Open();
                cmd.ExecuteNonQuery();
                con.Close();
            }
            catch (Exception ex)
            {
                Response.Write(ex.Message);
            }
            
        }
        }

protected void btnShow_Click(object sender, EventArgs e)
    {
        SqlCommand cmd=new SqlCommand("select * from userimage where id="+8 ,con);
        con.Open();
        SqlDataReader dr = cmd.ExecuteReader();
        while (dr.Read())
        {
            Label1.Text = dr["FileName"].ToString();
            if (!string.IsNullOrEmpty(Convert.ToString(dr["FilePath"])))
            {
              Image1.ImageUrl = "~/Ads_Images/" + Convert.ToString(dr["FilePath"]);
            }
        }
        con.Close();
    }

The image is also not showing when i try to retreive through data reader, but when i debug code its fetching proper image value at imageurl.

There is some problem in folder(Ads_Images), It stores image in it but not fetching image from it, if i using the folder(Images) and try to store and fetch image then it works fine.
Anyone knows, why it happens???
I Dont want to store all user images with my Images folder.