RadioButtonList rbl = new RadioButtonList();
rbl.ID = "rblID";
rbl.AutopostBack="true";
rbl.Items.Add(new ListItem("One", "1"));
rbl.Items.Add(new ListItem("Two", "2"));
rbl.Items.Add(new ListItem("Three", "3"));
rbl.DataBind();
PlaceHolder1.Controls.Add(rbl);
RadioButtonList rb = (RadioButtonList)FindControl("rblID");
TextBox1.Text = rb.SelectedValue;