You have to save your file at that folder by writing this code :
if (FileUpload1.HasFile == true)
{
FileUpload1.SaveAs(Server.MapPath("~/Traditional Day 2012/Images/" + FileUpload1.FileName));
string ImageUrl = "~/Traditional Day 2012/Images/" + FileUpload1.FileName;
}
And then You just have to bind that url in your ImageButton Control....