This will help you.
<div id="seletedItems" style="display:none"></div>
<asp:DropDownList ID="DropDownList1" runat="server" onchange="Select();">
<asp:ListItem>Select</asp:ListItem>
<asp:ListItem>1</asp:ListItem>
<asp:ListItem>2</asp:ListItem>
<asp:ListItem>3</asp:ListItem>
<asp:ListItem>4</asp:ListItem>
<asp:ListItem>5</asp:ListItem>
</asp:DropDownList>
<script type="text/javascript">
function Select() {
var item = document.getElementById("DropDownList1").value;
if (item != "Select") {
var selected = document.getElementById("seletedItems").innerHTML;
if (selected.search(item) == -1) {
document.getElementById("seletedItems").innerHTML += item + ",";
}
else {
alert("item already selected");
}
}
}
</script>