<html xmlns="http://www.w3.org/1999/xhtml">
<head>
<title>Untitled Page</title>
<script type="text/javascript">
function clk()
{
document.getElementById('Button1').click();
}
</script>
</head>
<body onload="clk();">
<div>
<form id="form1" runat="server">
<div>
<asp:Button ID="Button1" runat="server" Text="Choose file.."
onclick="Button1_Click" />
<br />
<asp:TextBox ID="TextBox1" runat="server"></asp:TextBox>
</div>
</form>
</div>
</body>
</html>
In the Above code i have called a java script function which perform click event of Asp button during page load.
I hope this may help u...