If you would like to add a sub-node to the node, try this:
string ItemName = "MTP/2 -3-PLY";
string subitemname = "op 1";
TreeNode node = new TreeNode(ItemName);
node.Nodes.Add(subitemname);
treeView1.Nodes.Add(node);
In case you want to add a childnode to the existing node, use this:
var node = treeView1.Nodes[0];
node.Nodes.Add(subitemname);